Composite

Part:BBa_K4644016:Design

Designed by: Han Cao   Group: iGEM23_BUCT   (2023-10-09)


pZE-glnAp2-ttgR-asrG-Pttg-bsrG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1936
    Illegal SpeI site found at 1879
    Illegal PstI site found at 875
    Illegal PstI site found at 1354
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1936
    Illegal SpeI site found at 1879
    Illegal PstI site found at 875
    Illegal PstI site found at 1354
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1936
    Illegal BamHI site found at 2080
    Illegal XhoI site found at 150
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1936
    Illegal SpeI site found at 1879
    Illegal PstI site found at 875
    Illegal PstI site found at 1354
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1936
    Illegal SpeI site found at 1879
    Illegal PstI site found at 875
    Illegal PstI site found at 1354
    Illegal AgeI site found at 1663
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We obtained the corresponding gene sequences from the genomes of Pseudomonas putida and Bacillus subtilis (primer sequences provided in the supporting information). Using the pZe plasmid as a vector, we successfully constructed the pathway.

Source

Bacillus subtilis strain A-5

References

Tian Z X, Li Q S, Buck M, et al. The CRP–cAMP complex and downregulation of the glnAp2 promoter provides a novel regulatory linkage between carbon metabolism and nitrogen assimilation in Escherichia coli[J]. Molecular microbiology, 2001, 41(4): 911-924.

Jahn N, Preis H, Wiedemann C, et al. BsrG/SR4 from Bacillus subtilis–the first temperature‐dependent type I toxin–antitoxin system[J]. Molecular microbiology, 2012, 83(3): 579-598.